Abstract: This paper discusses the characteristics and genesis of Daliuhang gold deposit in Jiaodong. The samples from several ore bodies of Daliuhang are collected and analyzed by electron microprobe, spectral quantification, fluid inclusions, and sulfur-carbon-hydrogen-oxygen isotopes from the aspects of geological characteristics, ore-forming fluid sources and material sources. It is considered that the Daliuhang gold deposit can be divided into three metallogenic stages. The ore-forming fluid is a mixture of meteoric water and magmatic water, rich in H₂O and CO₂.The ore-forming material may mainly derive from mantle, mixed with meteoric water and characterized by mixed crust mantle source. Daliuhang gold deposit belongs to medium plutonic deposits with low salinity, low density, and medium-low temperature.